DSpace Repository

Cytotoxicity of mangrove rhizhopora mucronate bark extracts as intracanal medicament

Show simple item record

dc.contributor.author cevanti, twi agnita
dc.date.accessioned 2020-04-20T12:36:24Z
dc.date.available 2020-04-20T12:36:24Z
dc.date.issued 2017-08-10
dc.identifier.uri http://dspace.hangtuah.ac.id/xmlui/handle/dx/998
dc.description.abstract Objectives : This study aims to determine the cytotoxicity of Rhizophora mucronata bark extracts as an intracanal medicament. Materials and Methods: Gingival mesenchymal stem cells (GMSCs) were exposed to the Rhizophora mucronata bark extracts at different concentrations of 0.5% (Group 1), 1% (Group 2), 2.5% (Group 3), 5% (Group 4), 10% (Group 5), and 20% (Group 6) to assess its cytotoxic effect. In addition, there were two control groups; one containing only the culture medium while another only with the cells. Cell viability was assessed by 3-(4, 5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This was done by adding MTT solution into each well after 3 minutes and then incubating them for 4 hours. Dimethylsulfoxide (DMSO) was then added to each well and shaken to mix. The absorbance was then measured on an ELISA plate reader with a reference wavelength of 630 nm. The extract at that said concentration is identified to be non-toxic if the cell viability is ≥ 50 %. Result: There was significant difference in cell viability of the GMSCs at various concentrations of the extract. The highest cell viability was found in group of cells which was treated with 1% concentration of Rhizopora Mucronata extract, while the with 10% concentration Rhizopora Mucronata significantly reduced the cell viability to the lowest among all groups. Conclusion: Rhizophora mucronata bark extracts at concentrations of 0.5%, 1%, 2.5%, and 5% can be used safely as endodontic intracanal medicament. en_US
dc.description.sponsorship hangtuah university en_US
dc.language.iso en_US en_US
dc.publisher Chinese Taipei association for dental science, SEEADE – South east asia association for dental education en_US
dc.subject Rhizophora mucronata, cytotoxicity, intracanal medicament en_US
dc.title Cytotoxicity of mangrove rhizhopora mucronate bark extracts as intracanal medicament en_US
dc.type Presentation en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search


My Account