Fakultas Kedokteran Gigi

Lev el of c reactive protein (CRP) among cardiovascular disease patients with chronic periodontitis in Cardiovascular Department of Dr. Soetomo National Hospital Surabaya

Fri, 08 Nov 2013 00:00:00 GMT

Lev el of c reactive protein (CRP) among cardiovascular disease patients with chronic periodontitis in Cardiovascular Department of Dr. Soetomo National Hospital Surabaya Wedarti, Yoifah Rizka Background: Periodontitis is a local inflammatory process mediating destruction of periodontal tissues triggered by bacterial insult. However, this disease is also characterized by systemic inflammatory host responses that may contribute, in part, to the recently reported higher risk for cardiovascular disease (CVD) among patients with periodontitis. Moderate elevation of C-reactive protein (CRP) has been found to be a predictor of increased risk for CVD. Elevated CRP levels in periodontal patients have been reported by several groups. In this study, we examined whether CRP plasma levels are increased in periodontitis and if there is a relation to severity of periodontal disease. Methods: CRP serum levels were assessed using reagen Immulite C-Reactive Protein ® / Immulite ® 1000 high-sensitivity CRP as a solid-phase, chemiluminescent immunometric assay in 100 subjects which diagnosed cardiovascular disease with chronic periodontitis. Periodontal Disease Index (PDI) were measured by clinical attachment loss, probing depths, and gingiva recession at six selection teeth : 16, 21, 24, 36, 41,44 known as Ramfjord teeth. Results: The measurement of Periodontal Disease Index (PDI) in 100 subjects with Cardiovascular Disease are Mild periodontitis (6,1%), Moderate periodontitis (70,4%), Severe periodontitis (23,5%) and only 2% with normal periodontal tissue. Increases in CRP levels were observed in these subjects. Subjects with high levels of clinical attachment loss (Severe periodontitis) had significantly higher mean of CRP levels (7,522 ± 0,3580 mg/L), moderate periodontitis are (5,284 ± 0,3138 mg/L) CRP levels and (2,017 ± 0,3656 mg/L) CRP levels for mild periodontitis Conclusions: The positive correlation between CRP and periodontal disease might be a possible underlying pathway in the association between periodontal disease which a chronic inflammation as one of risk factor for Cardiovascular Disease in these patients.

Combination of Anadara Granosa Shell-Stichopus Hermanni Gel on Osteoblast- Osteoclast and Blood Vessels in Femur Healing

Wed, 01 Jan 2020 00:00:00 GMT

Combination of Anadara Granosa Shell-Stichopus Hermanni Gel on Osteoblast- Osteoclast and Blood Vessels in Femur Healing Sari, Rima Parwati; Nurlaily, Isnainy; Heryana, Rizky Putri; Fatmawati, Wahyu; Ashrin, Meinar Nur

POTENTIAL APPLICATION OF CHITOSAN SYNTHESIZED FROM PENAEUS MONODON AS A DIRECT PULP CAPPING MATERIAL

Fri, 01 Jan 2021 00:00:00 GMT

POTENTIAL APPLICATION OF CHITOSAN SYNTHESIZED FROM PENAEUS MONODON AS A DIRECT PULP CAPPING MATERIAL Prananingrum, Widyasri; Sularsih; Revianti, Syamsulina; Prameswari, Noengki Chitosan could facilitate migration, proliferation and differentiation of pulp progenitor cells. The purpose of this study was to investigate the potential application of chitosan synthesized fromPenaeus monodonshells, as a direct pulp capping material. Chitosan was synthesized byPenaeus monodonshells using deproteinase, demineralisation, depigmentation and deacetylation methods. The molar teeth of the maleRattus norvegicus-strain-Wistar had been prepared for class one in the occlusal part and were divided into two groups, namely Group one (G1) applied Ca (OH)2 and Group two (G2) applied chitosan. Then, the histopathological evaluation was performed to observe odontoblast-like cells with hematoxylin eosin staining after seven and then 28 days following treatment. Measurement of the yield value, water content, molecular weight, deacetylation degree and cytotoxicity tests were carried out to determine the quality standards of chitosan. Statistics analysis used one-way analysis of variance (ANOVA) followed by an least significant different (LSD) post hoc test with multiple comparisons (p<0.05). This study showed that the yield value was 0.08%; water content was 8.73 %; molecular weight was 3012.83 g/mol; deacetylation degree was 86.22% and the cells viability was 97.83%. The application of chitosan was showed a significant difference in odontoblast-like cells on the seventh day of observation (p=0.001; p<0.05) and the 28th day of observation (p=0.002; p<0.05). Chitosan synthesized fromPenaeus monodon shells from Indonesia has strong potentials as a direct pulp capping material.

The Astaxanthin Effect On The Differential Count Of Inflamatory Cell In Nickel Allergy Balb/c Mice Model

Sun, 01 Aug 2021 00:00:00 GMT

The Astaxanthin Effect On The Differential Count Of Inflamatory Cell In Nickel Allergy Balb/c Mice Model Andriani, Dwi Nickel is a material that commonly used in dentistry, but in some individuals, it can cause type IV delayed-type hypersensitivity (DTH) reaction. In this case, DTH reaction pathway involves T cell helper 1 (Th1) activation that stimulates macrophages to release pro-inflammatory cytokine. Meanwhile, astaxanthin (AST) is carotenoid from red algae that has an anti-inflammatory effect by inhibiting inflammatory mediators. Hematology evaluation is one of the evaluation methods for allergies. This investigation aimed to determine the effect of astaxanthin supplementation on the differential count of inflammatory cells in nickel allergy of mice model. Sixteen Balb/C mice were randomly divided into 4 groups: normal group (N), without therapy group (NA), Astaxanthin 12 mg treatment group (Ast12) and Astaxanthin 6 mg treatment group (Ast6). All groups were given an injection of Nickel(II) chloride (NiCl2 ), Complete Freud’s Adjuvant (CFA), and Incomplete Freud’s Adjuvant (IFA) to obtain the nickel allergy model. Analysis of differential count in an inflammatory cell (neutrophil, lymphocyte, monocyte and eosinophil) performed by utilizing flow cytometry, then, the data were analyzed using Univariate Analysis (p<0.05). As the result, there were no any significant differences between groups for neutrophil and lymphocyte showed by One-Way ANOVA test. Kruskal-Wallis test for eosinophil and monocyte also exhibited no significant difference. In sum, astaxanthin supplementation did not have any effect on the differential count of inflammatory cells (neutrophil, lymphocyte, monocyte and eosinophil) in nickel allergy mice model compared to the normal and without therapy ones

Direct composite restoration using stamp technique and pizza technique: A case report

Fri, 01 May 2020 00:00:00 GMT

Direct composite restoration using stamp technique and pizza technique: A case report Soesilo, Diana; Wijaya, Yongki Hadinata; Pangabdian, Fani; Rochyani, Linda Introduction: The purpose of a restoration is to restore the shape, function, and phonetics and aesthetics of damaged teeth. The disadvantage of direct composite restorations is the formation of dental anatomical structures that require operator skills so that they can optimally restore tooth occlusion. Case report: Case 1: A 21-year-old female patient with D4 caries, Site 1 size 2 on tooth number 37 and the patient's dental diagnosis was Reversible Pulpitis. The patient was treated with a composite restoration using the stamp technique. Case 2: A 50-year-old male the patient with D5 caries, Site 1 Size 2 on tooth number 46 and patient’s dental diagnosis was Irreversible Pulpitis. The patient was treated with a composite restoration using the pizza technique. Conclusion: Stamp technique is indicated for hidden caries in pits and fissures or proximal parts of the tooth with intact anatomical forms. The pizza technique can be used in caries with incomplete anatomical forms but requires operator skills to form biomimetic dental anatomy.

The effect of the release behavior of simvastatin from different PLGA particles on bone regeneration in vitro and in vivo: Comparison of simvastatin-loaded PLGA microspheres and nanospheres

Tue, 01 Mar 2016 00:00:00 GMT

The effect of the release behavior of simvastatin from different PLGA particles on bone regeneration in vitro and in vivo: Comparison of simvastatin-loaded PLGA microspheres and nanospheres Prananingrum, Widyasri Simvastatin is a cholesterol-lowering drug known to affect bone formation in vivo and its sustainable administration into localized areas is of particular interest in the dental field. Two simvastatin-loaded poly(lactic-co-glycolic acid) (PLGA) formulations (PLGA microspheres and PLGA nanospheres) were compared to investigate whether a sustainable supply of simvastatin from a PLGA-based carrier is effective for bone regeneration. PLGA microspheres successfully presented sustained release of simvastatin for one month, whereas simvastatin was continuously released from PLGA nanospheres for one week. The difference of drug release pattern between two PLGA particles was confirmed by Korsmeyer ePeppas mathematical model. PLGA microspheres and simvastatin alone (no carrier) induced the proliferation MC3T3-E1 cells and increased alkaline phosphatase (ALP) activity, a differentiation marker of MC3T3-E1 cells, whereas PLGA nanospheres did not. These results suggested that PLGA nanospheres had an adverse effect on bone generation in vitro due to the production of PLGA metabolized products. Both PLGA microspheres and PLGA nanospheres provided a bone formation effect in an in vivo rat calvaria bone defect model and PLGA microspheres were superior to PLGA nanospheres. Our data suggest that simvastatin-loaded PLGA microspheres can release simvastatin sustainably and induce bone formation more efficiently than PLGA nanospheres, thus promoting bone regeneration.

Controlled release of clarithromycin from PLGA microspheres enhances bone regeneration in rabbit calvaria defects

Tue, 01 Nov 2016 00:00:00 GMT

Controlled release of clarithromycin from PLGA microspheres enhances bone regeneration in rabbit calvaria defects Prananingrum, Widyasari This study evaluated the sustained release effect of clarithromycin-loaded in PLGA microspheres in a rabbit calvaria defect model. Four bone defects (ø5.0) were created in the calvaria of New Zealand White rabbits (n521, n57/time point). The defects were randomly designated to four groups. Group 1: No augmentation (sham), Group 2: beta-tricalcium phosphate (b-TCP), Group 3: b-TCP with 0.12 mg clarithromycin, and Group 4: b-TCP with 6.12 mg PLGAmicrospheres loaded with 0.12 mg Clarithromycin. After 2, 4, and 12 weeks of healing, bone regeneration was evaluated using micro-computed tomography (mCT) and histology. Clarithromycin release from PLGA microspheres revealed sustained release for around 4 weeks with 50% release during the first week. Histologically, new bone formation was evident at 2 and 4 weeks of healing in all groups and bone formation increased as a function of healing time. At 12 weeks, Group 4 showed significantly higher amount of newly formed bone compared to Group 1. The mCT showed that Group 4 expressed significantly higher bone formation compared to Group 1 at all time points. The in vivo findings showed that b-TCP with clarithromycin-loaded microspheres can enhance bone formation in bone defects.

Application ofporoustitaniuminprosthesis production usingamoldlessprocess:Evaluation of physicalandmechanicalpropertieswithvarious particle sizes,shapes,andmixingratios

Fri, 01 Apr 2016 00:00:00 GMT

Application ofporoustitaniuminprosthesis production usingamoldlessprocess:Evaluation of physicalandmechanicalpropertieswithvarious particle sizes,shapes,andmixingratios Prananingrum, Widyasri he prostheticapplicationsoftitaniumhavebeenchallengingbecausetitaniumdoesnot possess suitablepropertiesfortheconventionalcastingmethodusingthelostwax technique.Wehavedevelopedaproductionmethodforbiomedicalapplicationofporous titanium usingamoldlessprocess.Thisstudyaimedtoevaluatethephysicaland mechanical propertiesofporoustitaniumusingvariousparticlesizes,shapes,andmixing ratio oftitaniumpowdertowaxbinderforuseinprosthesisproduction.CPTipowders with differentparticlesizes,shapes,andmixingratiosweredividedinto five groups. A 90:10wt%mixtureoftitaniumpowderandwaxbinderwaspreparedmanuallyat70 1C. After debindingat380 1C, thespecimenwassinteredinArat1100 1C withoutamoldfor 1 h.Thelinearshrinkageratioofsinteredspecimensrangedfrom2.5%to14.2%.Thelinear shrinkage ratioincreasedwithdecreasingparticlesize.Whilethelinearshrinkageratioof Groups 3,4,and5wereapproximately2%,Group1showedthehighestshrinkageofall. The bendingstrengthrangedfrom106to428MPaundertheinfluence ofporosity.Groups 1 and2presentedlowporosityfollowedbyhigherstrength.Theshearbondstrength ranged from32to100MPa.Theshearbondstrengthwasalsoparticle-sizedependent.The decrease intheporosityincreasedthelinearshrinkageratioandbendingstrength. Shrinkage andmechanicalstrengthrequiredforprosthesesweredependentontheparticle size andshapeoftitaniumpowders.These findings suggestedthatthisproductionmethod can beappliedtotheprostheticframeworkbyselectingthematerialdesign.

Bone ingrowth of various porous titanium scaffolds produced by a moldless and space holder technique: an in vivo study in rabbits

Tue, 01 Nov 2011 00:00:00 GMT

Bone ingrowth of various porous titanium scaffolds produced by a moldless and space holder technique: an in vivo study in rabbits Prananingrum, Widyasri Porous titanium has long been desired as a bone substitute material because of its ability to reduce the stress shielding in supporting bone. In order to achieve the various pore structures, we have evolved a moldless process combined with a space holder technique to fabricate porous titanium. This study aims to evaluate which pore size is most suitable for bone regeneration using our process. The mixture comprising Ti powder, wax binder and PMMA spacer was prepared manually at 70 °C which depended on the mixing ratio of each group. Group 1 had an average pore size of 60 μm, group 2 had a maximum pore size of 100 μm, group 3 had a maximum pore size of 200 μm and group 4 had a maximum pore size of 600 μm. These specimens were implanted into rabbit calvaria for three and 20 weeks. Thereafter, histomorphometrical evaluation was performed. In the histomorphometrical evaluation after three weeks, the group with a 600 μm pore size showed a tendency to greater bone ingrowth. However, after 20 weeks the group with a pore size of 100 μm showed significantly greater bone ingrowth than the other groups. This study suggested that bone regeneration into porous titanium scaffolds is pore size-dependent, while bone ingrowth was most prominent for the group with 100 μm-sized pores after 20 weeks in vivo.

Antibacterial activity of sea cucumber extract to porphyromonas gingivalis in vitro

Tue, 01 Jan 2013 00:00:00 GMT

Antibacterial activity of sea cucumber extract to porphyromonas gingivalis in vitro Mulawarmanti, Dian; Parihsini, Kristanti; Wedarti, Yoifah Rizka

Prosthetic Rehabilitation of a Partially Edentulous Patient with Chronic Periodontitis

Mon, 01 Aug 2016 00:00:00 GMT

Prosthetic Rehabilitation of a Partially Edentulous Patient with Chronic Periodontitis Diyah, Chaterina Background: One of the most common inflammation disease in the oral cavity for the past few years is the chronic form of periodontitis. Patients with chronic periodontitis, who her jobs requires to interact with people are a real challenge for the dentist and the prosthetis. The problem with the traditional method is the fact that for rehabilitation of patients who have lost their tooth/teeth, has to be waited for approximately 8 to 12 weeks before having their dentures. There are many advantages of immediate as opposed to conventional complete denture. Hence, dentists have no opportunity to observe the anterior teeth at the try in appointment; therefore the esthetic result cannot be evaluated until the dentures are inserted. Purpose: The prosthetic rehabilitation of a partially edentulous patient with chronic periodontitis using immediate maxillary full denture and immediate mandibular partial overdenture. Case and Case management: We presented a patient suffered form chronic periodontitis with major complain of her teeth mobility, mostly anterior teeth. She had undergone periodontal treatment, but the result were bad. In maxilla, at the end of periodontal treatment, all of her teeth need to be extracted and replaced by dentures. In mandible, there were some teeth that can be used as abutment for an overdenture, so we decided to place immediate partial overdenture in mandible with consideration both of her functional and esthetic aspects. Conclusion: One of the advantages of immediate dentures is patients don't have to ben in edentulous state for any length of time. After treatment, patient was evaluated, fortunately she had no complaints and was happy with her new smile.

Prosthetic Rehabilitation of a Partially Edentulous Patient with Chronic Periodontitis

Mon, 29 Aug 2016 00:00:00 GMT

Prosthetic Rehabilitation of a Partially Edentulous Patient with Chronic Periodontitis Diyah Nanik, Chaterina Background: One of the most common inflammation disease in the oral cavity for the past few years is the chronic form of periodontitis. Patients with chronic periodontitis, who her jobs requires to interact with people are a real challenge for the dentist and the prosthetis. The problem with the traditional method is the fact that for rehabilitation of patients who have lost their tooth/teeth , has to be waited for approximately 8 to 12 weeks before having their dentures. There are many advantages of immediate as opposed to conventional complete denture. Hence, dentists have no opportunity to observe the anterior teeth at the try in appointment; therefore, the esthetic result cannot be evaluated until the dentures are inserted. Purpose: The prosthetic rehabilitation of a partially edentulous patient with chronic periodontitis using immediate maxillary full denture and immediate mandibular partial overdenture. Case and Case management: We presented a patient suffered from chronic periodontitis with major complain of her teeth mobility, mostly anterior teeth. She had undergone periodontal treatment, but the result were bad. In maxilla, at the end of peridontal treatment, all of her teeth need to be extracted and replaced by dentures. In mandible, there were some teeth that can be used as abutment for an overdenture, so we decided to place immediate partial overdenture in mandible with consideration both of her functional and esthetic aspects. Conclusion: One of the advantages of immediate dentures is patients don’t have to be in edentulous state for any length of time. After treatment, patient was evaluated, fortunately she had no complaints and was happy with her new smile.

Prosthetic Rehabilitation of a Partially Edentulous Patient with Chronic Periodontitis

Fri, 26 Aug 2016 00:00:00 GMT

Prosthetic Rehabilitation of a Partially Edentulous Patient with Chronic Periodontitis Diyah Nanik, Chaterina Background: One of the most common inflammation disease in the oral cavity for the past few years is the chronic form of periodontitis. Patients with chronic periodontitis, who her jobs requires to interact with people are a real challenge for the dentist and the prosthetis. The problem with the traditional method is the fact that for rehabilitation of patients who have lost their tooth/teeth, has to be waited for approximately 8 to 12 weeks before having their dentures. There are many advantages of immediate as opposed to conventional complete denture. Hence, dentists have no opportunity to observe the anterior teeth at the try in appointment; therefore, the esthetic result cannot be evaluated until the dentures are inserted. Purpose: The prosthetic rehabilitation of a partially edentulous patient with chronic periodontitis using immediate maxillary full denture and immediate mandibular partial overdenture. Case and case management: We presented a patient suffered from chronic periodontitis with major complain of her teeth mobility, mostly her anterior teeth. She had undergone periodontal treatment, but the result were bad. In maxilla, at the end of periodontal treatment, all of her teeth need to be extracted and replaced by denture. In mandible, there were some teeth that can be used as abutment for an overdenture, so we decided to place immediate partial overdenture in mandible with consideration both of her functional and esthetic aspects. Conclusion: One of the advantages of immediate denture is patients don't have to be in edentulous state for any length of time. After treatment, patient was evaluated, fortunately she had no complaints and was happy with her new smile.

Dental Public Health Warrior Project : Attempt to Increase Awareness of Preschool Children's Dental Health

Mon, 01 Apr 2019 00:00:00 GMT

Dental Public Health Warrior Project : Attempt to Increase Awareness of Preschool Children's Dental Health Aulia Dwi, Maharani The Dental Public Health Warrior (DPHW) project is a social activity which provides a creative,fun, and interactive dental health education throughout kindergarten and elementary schools inKeputih-Surabaya, Indonesia. The aim of this study was to determine the effectivity of those projects in increasing the awarness of preschool children’s dental health. This is a pre experimental study, the ethical approval for this study has been obtained. Subjects are 462 children age 4–5 years in Keputih from 2015 to 2017. All subjects were educated by the DPHW team, pretest and post test were performed and and def-t score was collected by a calibrated dentist at the same time. Def-t data were analyzed by Kruskal-Wallis and Mann-Whitney tests. The percentage of children with free caries decrease in 2016, (from 3.3% to 3,2%) and increase in 2017 (8,3%). There’s significant difference among def-t variable (p= 0,101). There is signficant difference from pre-test to post-test in 2015 (p= 0,006), 2016 (p=0,001) and 2017 (p=0,002). The DPHW can be effectively used to increase the awareness of preschool children’s dental health in KeputihSurabaya, in Indonesia.

Expression Of Runx2 And Osteoblast Cell On The Periodontal Of Diabetes Mellitus Wistar Rat With Diet Extract Lemuru Fish Oils Treatment

Thu, 10 Jan 2019 00:00:00 GMT

Expression Of Runx2 And Osteoblast Cell On The Periodontal Of Diabetes Mellitus Wistar Rat With Diet Extract Lemuru Fish Oils Treatment Damaiyanti, Dian Widya; Mulawarmanti, Dian; Parisihni, Kristanti; Widyastuti; Kurniawan, Hansen Abstract. The purpose of this study was to find an innovative strategy for controling progresivity of bone destruction on periodontal that caused by diabetic condition. Methods: Wistar rats sample are devided into 4 group; negative control group, 3 group with lemuru fish oil supplementation (4ml/kg of weight t, 8ml/kg of weight and 16ml/kg of weight). One week before treatment all group induced with STZ 65ml/KgBB and nicotinamide 110ml/KgBB to produced diabetes conditions. Immunohistochemistry slide of periodontal tissue was prepare after 3 weeks therapy. RUNX2 was count using HSCORE index, and osteoblast cell amount was count using image raster by optilab program. Results: Statistical analyses demonstrated a significant increase of RUNX2 expression in negative control group compare to treatment group (p<0,05), and in treatment group showing less of RUNX2 expression (p<0,05). Meanwhile Osteoblast cell amount was found increased and has significant difference in group 8ml/kg among other groups (p<0,05) and lower osteoblast cell result in control group. The result lead to another mechanism that may involved in bone formation to induced osteoblast cell proliferation beside trancript factor RUNX2 which showed by treatment group of 8ml/kgWeight. Conclusion: The stimulation of osteoblast cell in diabetic condition can be induced by lemuru fish oil treatment, which regulated by another pathway mechanism beside transcript factor RUNX2

Novel Development of Phosphate Treated Porous Hydroxyapatite

Sun, 01 Jan 2017 00:00:00 GMT

Novel Development of Phosphate Treated Porous Hydroxyapatite Doi, Kazuya; Abe, Yasuhiko; Kobatake, Reiko; Okazaki, Yohei; Oki, Yoshifumi; Naito, Yoshihito; Prananingrum, Widyasri; Tsuga, Kazuhiro

PEEK with Reinforced Materials and Modifications for Dental Implant Applications

Sun, 01 Jan 2017 00:00:00 GMT

PEEK with Reinforced Materials and Modifications for Dental Implant Applications Rahmitasari, Fitria; Ishida, Yuichi; Kurahashi, Kosuke; Matsuda, Takashi; Watanabe, Megumi; Ichikawa, Tetsuo

A Critical Role for Thymic Stromal Lymphopoietin in Nickel-Induced Allergy in Mice

Sat, 01 Mar 2014 00:00:00 GMT

A Critical Role for Thymic Stromal Lymphopoietin in Nickel-Induced Allergy in Mice Ashrin et al., Meinar Nur

The Expression of Collagen Type-I in the Tension Area of Orthodontic Tooth Movement with Adjuvant of Hyperbaric Oxygen Therapy

Fri, 01 Jan 2016 00:00:00 GMT

The Expression of Collagen Type-I in the Tension Area of Orthodontic Tooth Movement with Adjuvant of Hyperbaric Oxygen Therapy Brahmanta, Arya; Soetjipto, Soetjipto; Narmada, I.B.