The Different Effect Between Fluoride Tooth Paste and Its Combination With Probiotics Lactobacillus Reuteri

Abstract

Objective: Fluoride is one of the most agents that prevent dental caries. Probiotics L.reuteri can reduce number of S.mutans and stimula te salivary sIgA. The objective of this study was to examine the effect of fluoride toothpaste and its combinations with probiotic L.reuteri in child salivary sIgA spesific S.mutans level.

Method: This study was quasi eksperimental with pre - test post - test control group design. 24 healthy children, 4 - 6 years old, devide into 2 groups. 12 free caries (group1) and 12 caries (group 2). Each group treated with fluoride toothpaste (sodium fluoride 0,11%) during 1 weeks and combine with probiotic L.reut eri prodentis chewing gum (2x10 8 CFU) during 2 weeks. Unstimulated saliva collected in the morning due to circadian rhytm, and responden instructed fasting for 2 hours before saliva collection. Salivary sIgA spesific S.mutans were measured with ELISA metho d. Data were analyzed with Kruskall Wallis and Mann Withney (p=0,05).

Result: There were significant differences of sIgA level between free caries and caries group before treated (p=0,017) and after 7 days treated with fluoride toothpaste (p=0,018). There were no significant differences of sIgA level between free caries and caries group after 7 days (p=0,198) and 14 days (p=0,456) treated with combination fluoride and probiotic toothpaste.

Conclusion: Salivary sIgA spesific S.mutans level in caries is sign ificantly higher than free caries before and after treated with fluoride toothpaste. There is no difference salivary sIgA spesific S.mutans level in caries and free caries group, before and after treated with fluoride toothpaste and probiotic L.reuter