Expression Of Runx2 And Osteoblast Cell On The Periodontal Of Diabetes Mellitus Wistar Rat With Diet Extract Lemuru Fish Oils Treatment

Abstract

Abstract. The purpose of this study was to find an innovative strategy for controling progresivity of bone destruction on periodontal that caused by diabetic condition. Methods: Wistar rats sample are devided into 4 group; negative control group, 3 group with lemuru fish oil supplementation (4ml/kg of weight t, 8ml/kg of weight and 16ml/kg of weight). One week before treatment all group induced with STZ 65ml/KgBB and nicotinamide 110ml/KgBB to produced diabetes conditions. Immunohistochemistry slide of periodontal tissue was prepare after 3 weeks therapy. RUNX2 was count using HSCORE index, and osteoblast cell amount was count using image raster by optilab program. Results: Statistical analyses demonstrated a significant increase of RUNX2 expression in negative control group compare to treatment group (p<0,05), and in treatment group showing less of RUNX2 expression (p<0,05). Meanwhile Osteoblast cell amount was found increased and has significant difference in group 8ml/kg among other groups (p<0,05) and lower osteoblast cell result in control group. The result lead to another mechanism that may involved in bone formation to induced osteoblast cell proliferation beside trancript factor RUNX2 which showed by treatment group of 8ml/kgWeight. Conclusion: The stimulation of osteoblast cell in diabetic condition can be induced by lemuru fish oil treatment, which regulated by another pathway mechanism beside transcript factor RUNX2