The Effects of High Pressure Oxygen Therapy in Osteocytes Alveolar Bone of Diabetes Mellitus Wistar Rat Induced by Porphyromonas Gingivalis

Abstract

: Periodontitis is one of oral complication that commonly found in patients with Diabetes Mellitus. One of the bacteria that causing this disease is Porphyromonas gingivalis (P.g). Handling periodontitis with Diabetes Mellitus does not include local factors only, but also systemic factors such as handling of blood sugar because therapy of periodontitis in patients with systemic disease also has the potential to improve overall general conditions. Mechanical periodontitis teraphy can be carried out by scaling and root planing (SRP) but the healing it does not indicate the new connective tissue attachment. High pressure oxygen teraphy (HBOT), teraphy with breathing using 100% oxygen in high pressure chamber (more than 1 ATA), can accelerate alveolar bone healing as part of health tissue regeneration Purpose: To prove the effects of high pressure oxygen teraphy in the increasing of alveolar bone osteocytes of rat with DM induced by P.g. Materials and Methods: This research adopts post test only control group design. Fifteen male wistar rats are divided into three groups. K- ( negative control group), K1: induced by P.g and Streptozotocin, and K2: induced by P.g, Streptozotocin, high pressure oxygen teraphy 2,4 ATA 100% O2 3x30 minutes with five minute interval of air inhalation for five consecutive days. Results: Kruskal-Wallis test (p<0,05) and Mann-Whitney U (p>0,05) shows decreasing of osteocytes of alveolar bone significantly in K1 (3,55±0,93) that compared with K0 (5,33±2,32), increasing significantly in K2 (5,40±1,51) that compared with K1 (3,55±0,93), and no main changes between K2 (5,40±1,51) and K0 (5,33±2,32). Conclusion: HBOT 2,4 ATA 100% O2 3x30’ with 5’ interval of air inhalation for five consecutive days can increase of alveolar bone osteocytes of rat with DM induced by P.g.