POTENTIAL APPLICATION OF CHITOSAN SYNTHESIZED FROM PENAEUS MONODON AS A DIRECT PULP CAPPING MATERIAL

Abstract

Chitosan could facilitate migration, proliferation and differentiation of pulp progenitor cells. The purpose of this study was to investigate the potential application of chitosan synthesized fromPenaeus monodonshells, as a direct pulp capping material. Chitosan was synthesized byPenaeus monodonshells using deproteinase, demineralisation, depigmentation and deacetylation methods. The molar teeth of the maleRattus norvegicus-strain-Wistar had been prepared for class one in the occlusal part and were divided into two groups, namely Group one (G1) applied Ca (OH)2 and Group two (G2) applied chitosan. Then, the histopathological evaluation was performed to observe odontoblast-like cells with hematoxylin eosin staining after seven and then 28 days following treatment. Measurement of the yield value, water content, molecular weight, deacetylation degree and cytotoxicity tests were carried out to determine the quality standards of chitosan. Statistics analysis used one-way analysis of variance (ANOVA) followed by an least significant different (LSD) post hoc test with multiple comparisons (p<0.05). This study showed that the yield value was 0.08%; water content was 8.73 %; molecular weight was 3012.83 g/mol; deacetylation degree was 86.22% and the cells viability was 97.83%. The application of chitosan was showed a significant difference in odontoblast-like cells on the seventh day of observation (p=0.001; p<0.05) and the 28th day of observation (p=0.002; p<0.05). Chitosan synthesized fromPenaeus monodon shells from Indonesia has strong potentials as a direct pulp capping material.