SITOTOKSISITAS EKSTRAK BIJI JINTEN HITAM (NIGELLA SATIVA) SEBAGAI BAHAN IRIGASI SALURAN AKAR

Abstract

Background : Along with the raise of economical level of the society, dental health awareness is also raised significantly. As dental science developed, teeth extraction is no longer a solution of injured teeth as long as it has solid supported tissue. Non vital teeth or necrotic teeth can be treated by endodontic root canal treatment by removing the pulp tissue both in pulp chamber and pulp canal. Cleaning and shaping are important stages in endodontic treatment. Irigation is needed in those stages to clean the canal from residual nectrotic tissue, dentin particles, microorganism and as irrigation to smooth the root canal preparation. It should be done because root canal preparation without irrigation will cause 70% residual debris in apical and wall of root canal. Irrigation solution spuld meet proper ctiteria that it should has antibacterial properties, dissolve the necrotic tissues, cleanse the debris in root canal and not toxic to periradicular tissue. Jinten hitam (Nigella sativa) seed extract has been used to be traditional herbs which has antibacterial effect. Purpose : The aim of this study is to examine the cytotoxicity of Jinten hitam (Nigella sativa) seed extract as root canal tooth irrigation. Material and Methods : Samples in cytotoxicity test were fibroblast cell (BHK-21) with culture test methods, treated by giving Jinten hitam seed extract in various concentration. Samples were divided into 5 groups, which were group 1 with concentration of 0,2 gr/ml, group 2 : 0,1 gr/ml, group 3 : 0,05 gr/ml, group 4 : 0,025 gr/ml, group 5 :control. MTT were added after 3 minutes, followed by incubation for 4 hours. DMSO solution were added, shaked, ten were examined by Elisa reaader in wave length of 630. Cytotoxicity were stated by examined the viable cells, when more then 60% were viable, it stated as non toxic. Result. Data were analyzed by non parametric test (Kruskal-Wallis Test). Result : showed 0.00 (p<0.005) means that no significance difference in cytotoxicity of various concentration of jinten hitam seed extract. Conclusion : There were no cytotoxicity effect of jinten hitam seed extract on concentration of 0.2 gr/ml, 0.1 gr/ml, 0.05 gr/ml dan 0.025 gr/ml. Conclusion : Jinten hitam seed extract has proved to have no cytotoxicity effect as root canal tooth irrigation solution, it could be considered as alternative irrigation material in root canal tooth irrigation.