Cytoxicity Test of Diadema Setosum Shell Extract Againts Fibroblast Culture Cell

Abstract

Background: Calcium is one of the basic ingredients of calcium hydroxide which is used in the field of dentistry as pulp capping material. Utilization of shells to be used as biomaterials Diadema setosum new to the field of dentistry, because one of the ingredients in Diadema setosum shells is calcium. Purpose: To find out the cytotoxicity of Diadema setosum shell extract against fibroblast cell cultures. Material and Methods: This research was carried out by using post test only control group design. Fibroblasts cultured in 96 wells were divided into a control group of cells (n=6), media control (n=6) and treatment (n=6). Treatment groups were given various doses extract with concentration of shells Diadema setosum 125 μg, 250 μg, 500 μg, 1000 μg, 2000 μg. Optical density was read with an ELISA reader and calculated the percentage of viability. The cell viability data were analyzed by One-way ANOVA statistical test and LSD. Result: Data indicate decreased cell viability in all treatment groups, level of concentration of the extract by Diadema setosum shell, that is 125 μg(83.7%), 250 μg(81.9%), 500 μg(66.25%), 1000 μg(57.08%), 2000 μg(42.95%). There is a significant difference (p=0.000) in all treatment groups after analyzed by using One-way ANOVA. Conclusion: Diadema setosum shell extract do not have toxic effects on cultured fibroblast cells at concentrations 125 μg, 250 μg, 500 μg, 1000 μg and has toxic effects on cultured fibroblast cells at the highest concentration, that is 2000 μg.