Abstract:
Background: Root canal treatment is the most common way to persist a tooth which has infected by root canal mixed bacteria. One of the root canal treatment stage is irrigation of root canal. Some studies reported that ozone water made from ozone generator has antibacterial activities against certain microorganism. In this study, ozone water, which is containing a lot of O3 has been explored as the antibacterial agent.
Objective: The aim of this study is to determine the antibacterial effect of ozone water to the growth of necrosis root canal bacterial.
Material and Method: Antimicrobial effect of ozone water in various concentration ( 0,1 ppm; 0,3 ppm; 0,6 ppm; 1 ppm ) to the growth of necrosis root canal bacteria were tested by dilution method in BHI broth, incubated anaerobically at 37°C for 24 hour. On the consecutive day, each treatment group along with positive and negative control group then continued to be inoculated in BHI agar by poured method and incubated anaerobically at 37°C for 24. The viable colony appeared were counted, stated in CFU/ml.
Result: Data were analyzed with ANOVA (one way) test and result showed the significant difference (p< 0.05) between all groups. LSD showed significant difference between group 0,3 ppm and 0,6 ppm. But no significant difference between group 0,1 ppm-0,3 ppm and 0,6 ppm-1ppm.
Conclusion: Ozone water has the antibacterial effect to the growth of necrosis root canal bacteria. The recommended concentration is 0,6 ppm.
